Outcome

This study highlights the significant potential of Hyperbaric Oxygen Therapy (HBOT) in enhancing bone regeneration particularly under conditions compromised by chronic diseases like Type 1 diabetes. HBOT improved early bone healing in diabetic rats and increased mast cell population in non-diabetic animals.

Introduction

Diabetes can significantly impair the body’s natural bone healing processes posing substantial challenges for individuals with bone injuries. This study investigates the impact of Hyperbaric Oxygen Therapy (HBOT) on bone regeneration in diabetic rats aiming to identify potential treatments for patients with Type 1 diabetes mellitus. HBOT involves breathing pure oxygen in a pressurized environment and has been previously suggested to aid in various healing processes. In this research twenty rats were divided into four groups: non-diabetic controls non-diabetic with HBOT treatment diabetic controls and diabetic with HBOT treatment. The findings revealed that HBOT significantly enhanced bone regeneration in non-diabetic rats and normalized bone healing in diabetic rats to levels comparable with non-diabetic controls. Additionally an increase in mast cell populations was observed in non-diabetic subjects following HBOT suggesting a possible role for these cells in facilitating bone repair mechanisms. This study provides promising insights into the potential of HBOT as a treatment to ameliorate bone healing issues related to diabetes.

Results

The study investigated the impact of Hyperbaric Oxygen Therapy (HBOT) on bone regeneration in diabetic rats. Twenty rats were divided into four groups: Control (C) Control with HBOT (CH) Diabetes (D) and Diabetes with HBOT (DH). Diabetes was induced in the diabetic groups and bone defects were created in the femurs of all the animals. HBOT was administered daily for seven days to the CH and DH groups.

Results showed that HBOT significantly enhanced bone regeneration in the non-diabetic control group (CH) with this group exhibiting more advanced bone regeneration and significantly greater bone formation compared to the other groups (p < 0.001). Diabetic rats without HBOT (D) demonstrated decreased bone formation in comparison to both non-diabetic groups (C and CH) (p < 0.001). However the diabetic group that received HBOT (DH) exhibited bone formation comparable to the control group (C) suggesting that HBOT effectively mitigated the diabetes-related impediments to bone healing (p > 0.05).

Additionally it was observed that the mast cell population significantly increased in the CH group compared to the other groups (p < 0.05). This indicates that HBOT may influence immune response mechanisms during bone healing in non-diabetic rats. Interestingly no significant difference in mast cell population was observed between the diabetic groups (D and DH).

In summary HBOT improved early bone regeneration in diabetic rats and resulted in a significant increase in mast cell population only in non-diabetic animals. These findings suggest the potential of HBOT as a therapeutic approach to reduce the adverse effects of diabetes on bone healing.

Conclusion

In conclusion this study underscores the promising potential of Hyperbaric Oxygen Therapy (HBOT) in enhancing bone regeneration particularly in the context of diabetes-related impairments. Key findings indicate that HBOT significantly improved early bone healing in diabetic rats aligning their regeneration levels with those of non-diabetic controls. The research also highlights an intriguing increase in mast cell populations in non-diabetic subjects following HBOT suggesting a possible immunological role in the bone healing process. These results affirm HBOT’s capability to mitigate the adverse effects of diabetes on bone regeneration. Looking forward further investigations are warranted to elucidate the underlying mechanisms of HBOT’s action and to refine treatment protocols paving the way for effective clinical applications in managing bone regeneration issues associated with diabetes.